Journal: BMC Cancer

Article Title: Nitric-Oxide Synthase trafficking inducer (NOSTRIN) is an emerging negative regulator of colon cancer progression

doi: 10.1186/s12885-022-09670-6

Figure Lengend Snippet: NOSTRIN expression is inversely related to aggressiveness of colon cancer cells. A Quantitative real time PCR analysis of Nostrin transcript in colon cancer cell lines HT29, HCT116. B Western blot analysis of NOSTRIN protein levels in colon cancer cell lines HT29, HCT116. The bar graph adjacent to the blot represents quantification of NOSTRIN protein levels normalized to endogenous control GAPDH using NIH ImageJ software. Full length blots are shown in Fig. S . C Photomicrograph of invaded HT29 and HCT116 cells from cell invasion assay. Scale bar: 100 μm, Magnification: 100X. D Invaded cell count per microscopic field. E. Colorimetric quantification of the invaded cells at the lower surface of the membrane ( n = 3). F Representative photo-micrographic images depicting wound closure ability of HT29 and HCT116 cell lines. Magnification used, 50X. G Quantification of percentage wound closure from a minimum of 5 measurements in each experiment using three different biological replicates. H Photomicrographs of colonies formed in soft agar by HT29 and HCT 116 cells. Scale bar: 100 μm, Magnification: 50X. I Fluorometric quantification of relative colony formation ability in soft agar of HT29 and HCT116 cells. Data is representative of three independent biological replicates. Error bars represent standard error of mean from three biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: To corroborate our ex vivo findings with in vivo colon cancer progression, we performed a real time PCR based human colon cancer cDNA array (OriGene, USA) containing cDNAs from different stages of colon cancer patients including those from normal colon samples, comparing changes in Nostrin transcripts among those patient samples.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Control, Software, Invasion Assay, Cell Counting, Membrane

Journal: BMC Cancer

Article Title: Nitric-Oxide Synthase trafficking inducer (NOSTRIN) is an emerging negative regulator of colon cancer progression

doi: 10.1186/s12885-022-09670-6

Figure Lengend Snippet: Real time PCR array analysis for profiling Epithelial-Mesenchymal transition (EMT) signature genes in NOSTRIN over-expressed colon cancer cell line. A Quantitative real time PCR analysis of Nostrin using RNA from empty vector transfected (control) and Nostrin cDNA transfected HCT116 cells. GAPDH was used as an endogenous control for normalization. B Western blot analysis of NOSTRIN using cell lysates from empty vector transfected (control) and Nostrin cDNA transfected HCT116 cells. GAPDH was used as an endogenous control. Full length blots are shown in Fig. S . Quantification of the protein level from the western blot was done using NIH ImageJ software and is represented using bar graphs adjacent to the blot; Error bars represent standard error of mean from three biological replicates. ** p < 0.01, *** p < 0.001. C Scatter plot of real time PCR array using control and Nostrin overexpressing HCT116 cells showing differential EMT gene expression pattern. Genes with similar expression patterns are represented by black dots that are close to the line of regression while yellow and blue dots are for the up-regulated and down-regulated genes, respectively. D Functional annotation of 16 genes that are differentially regulated in all three experiments upon over-expression of NOSTRIN in HCT116 cells

Article Snippet: To corroborate our ex vivo findings with in vivo colon cancer progression, we performed a real time PCR based human colon cancer cDNA array (OriGene, USA) containing cDNAs from different stages of colon cancer patients including those from normal colon samples, comparing changes in Nostrin transcripts among those patient samples.

Techniques: Real-time Polymerase Chain Reaction, Plasmid Preparation, Transfection, Control, Western Blot, Software, Gene Expression, Expressing, Functional Assay, Over Expression

Journal: BMC Cancer

Article Title: Nitric-Oxide Synthase trafficking inducer (NOSTRIN) is an emerging negative regulator of colon cancer progression

doi: 10.1186/s12885-022-09670-6

Figure Lengend Snippet: NOSTRIN down-regulation potentiates the metastatic potential of HT29 cells. A Quantitative real time PCR analysis of Nostrin using RNA from HT29 cells transfected with either scramble or a cocktail of two Nostrin siRNAs (50 nM or 100 nM). B Quantitative real time PCR analysis of Vimentin (Vim) and Moesin ( Msn ) using RNA from HT29 cells transfected with either scramble or 50 nM Nostrin siRNA cocktail. GAPDH was used as an endogenous control for normalization. C Photo-micrographic images of invaded HT29 cells transfected with either scramble or 50 nM Nostrin siRNA cocktail from cell invasion assay. Scale bar: 100 μm, Magnification: 100X. D Invaded cell count per microscopic field. E Colorimetric quantification of the invaded cells ( n = 3). F Representative photo-micrograph depicting wound closure ability of HT29 cells transfected with either scramble or 50 nM Nostrin siRNA cocktail. Magnification used, 50X. G Quantification of percentage wound closure 24 h post scratching from a minimum of 5 measurements in each experiment using three different biological replicates. H Western-blot analysis of EMT-associated proteins in control and NOSTRIN down-regulated HT29 cells. Full length blots are shown in Fig. S . I Quantification of the proteins relative to GAPDH using three biological replicates using NIH ImageJ software. Error bars represent standard error of mean from three biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: To corroborate our ex vivo findings with in vivo colon cancer progression, we performed a real time PCR based human colon cancer cDNA array (OriGene, USA) containing cDNAs from different stages of colon cancer patients including those from normal colon samples, comparing changes in Nostrin transcripts among those patient samples.

Techniques: Real-time Polymerase Chain Reaction, Transfection, Control, Invasion Assay, Cell Counting, Western Blot, Software

Journal: Scientific Reports

Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

doi: 10.1038/s41598-021-97559-y

Figure Lengend Snippet: The layout of the DNA microarray method module; each detection panel includes 4 detection modules, which can detect two specimens at the same time. Modules 1 and 3 are used to detect mutations in the rpoB gene, and modules 2 and 4 are used to detect mutations in the katG gene and inhA promoter. QC quality control probe; EC external control probe; BC blank control; NC negative control probe; IC internal control probe; WT wild-type.

Article Snippet: CapitalBio (Beijing, China) developed a DNA microarray chip method based on a variety of molecular analyses with PCR and reverse hybridization to detect drug resistance in TB bacteria .

Techniques: Microarray, Control, Negative Control

Journal: Scientific Reports

Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

doi: 10.1038/s41598-021-97559-y

Figure Lengend Snippet: The drug susceptibility test was used as a standard method to evaluate the efficacy of the DNA microarray for detecting RIF and INH resistance and MDR-TB.

Article Snippet: CapitalBio (Beijing, China) developed a DNA microarray chip method based on a variety of molecular analyses with PCR and reverse hybridization to detect drug resistance in TB bacteria .

Techniques: Microarray

Journal: Scientific Reports

Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

doi: 10.1038/s41598-021-97559-y

Figure Lengend Snippet: Comparison of the diagnostic efficacy of the DNA microarray method when sputum smear grades were ≤ 1 + and ≥ 2 +

Article Snippet: CapitalBio (Beijing, China) developed a DNA microarray chip method based on a variety of molecular analyses with PCR and reverse hybridization to detect drug resistance in TB bacteria .

Techniques: Comparison, Diagnostic Assay, Microarray

Journal: Scientific Reports

Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

doi: 10.1038/s41598-021-97559-y

Figure Lengend Snippet: Specimen processing procedure: A total of 5,911 sputum smear-positive specimens were collected. After experimental processing, 4148 specimens that were positive with the DNA microarray method and DST were finally included in the study. NTM, nontuberculous mycobacteria ; DST, drug sensitivity test.

Article Snippet: CapitalBio (Beijing, China) developed a DNA microarray chip method based on a variety of molecular analyses with PCR and reverse hybridization to detect drug resistance in TB bacteria .

Techniques: Microarray

Journal: Scientific Reports

Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

doi: 10.1038/s41598-021-97559-y

Figure Lengend Snippet: Pattern diagrams of several common drug-resistant gene mutations detected by the DNA microarray method. The white box is the detection site of the wild-type codon, and the red box is the site of the detected mutant codon. ( a ) rpoB gene Leu511Pro (CTG → CCG); ( b ) rpoB gene Asp516Tyr (GAC → TAC); ( c ) rpoB gene His526Tyr (CAC → TAC); ( d ) rpoB gene Ser531Trp (TCG → TGG); ( e ) rpoB gene Ser531Leu (TCG → TTG); ( f ) katG gene Ser315Thr (AGC → ACC); ( g ) katG gene Ser315Asn (AGC → AAC); ( h ) inhA gene promoter-15 (C → T).

Article Snippet: CapitalBio (Beijing, China) developed a DNA microarray chip method based on a variety of molecular analyses with PCR and reverse hybridization to detect drug resistance in TB bacteria .

Techniques: Microarray, Mutagenesis

Journal: Medical Journal of the Islamic Republic of Iran

Article Title: Human papilloma virus: A review study of epidemiology, carcinogenesis, diagnostic methods, and treatment of all HPV-related cancers

doi: 10.47176/mjiri.35.65

Figure Lengend Snippet: The classification of commercially available diagnostic techniques for the detection and genotyping of HPV

Article Snippet: , , PCR-based fluorescent bead array , ❖ Microarray-based HPV genotyping assays ○ The PapilloCheck HPV Screening Test(greiner bio), ○ Clart HPV 2 – papillomavirus clinical arrays( Genomica), ○ HPV GenoArray Test Kit ( Hybribio), ○ GeneTrack HPV DNA Chip( Daejeon), ○ GeneSQUARE HPV Microarray,( Kurabo) ○ INFINITI HPV-HR QUAD (AutoGenomics), ○ PANArray HPV Genotyping Chip( Panagene), ○ GG HPVCHIP ( Goodgene) ❖ Suspension array based HPV genotyping assays ○ The Multiplex HPV Genotyping Kit (Multimetrix, Heidelberg) ○ digene HPV Genotyping LQ Test(Qiagen).

Techniques: Diagnostic Assay, Amplification, Nested PCR, Multiplex PCR, Hybridization, Microarray, Suspension, Multiplex Assay, Real-time Polymerase Chain Reaction, HPV Assay, Southern Blot, In Situ Hybridization, Neutralization